| In vitro selection and evolution of functional proteins by using ribosome display J Hanes, A Plückthun Proceedings of the National Academy of Sciences 94 (10), 4937-4942, 1997 | 1719 | 1997 |
| Assembly of a Functional Immunoglobulin Fv Fragment in Escherichia coli A Skerra, A Plückthun Science 240 (4855), 1038-1041, 1988 | 1627 | 1988 |
| Fully synthetic human combinatorial antibody libraries (HuCAL) based on modular consensus frameworks and CDRs randomized with trinucleotides A Knappik, L Ge, A Honegger, P Pack, M Fischer, GÈ Wellnhofer, A Hoess, ... Journal of molecular biology 296 (1), 57-86, 2000 | 1140 | 2000 |
| Engineering novel binding proteins from nonimmunoglobulin domains HK Binz, P Amstutz, A Plückthun Nature biotechnology 23 (10), 1257-1268, 2005 | 893 | 2005 |
| Induction and exhaustion of lymphocytic choriomeningitis virus–specific cytotoxic T lymphocytes visualized using soluble tetrameric major histocompatibility complex class I … A Gallimore, A Glithero, A Godkin, AC Tissot, A Plückthun, T Elliott, ... The Journal of experimental medicine 187 (9), 1383-1393, 1998 | 812 | 1998 |
| High-affinity binders selected from designed ankyrin repeat protein libraries HK Binz, P Amstutz, A Kohl, MT Stumpp, C Briand, P Forrer, MG Grütter, ... Nature biotechnology 22 (5), 575-582, 2004 | 782 | 2004 |
| Stability engineering of antibody single-chain Fv fragments A Wörn, A Plückthun Journal of molecular biology 305 (5), 989-1010, 2001 | 756 | 2001 |
| In vitro generated antibodies specific for telomeric guanine-quadruplex DNA react with Stylonychia lemnae macronuclei C Schaffitzel, I Berger, J Postberg, J Hanes, HJ Lipps, A Plückthun Proceedings of the National Academy of Sciences 98 (15), 8572-8577, 2001 | 747 | 2001 |
| A comparison of strategies to stabilize immunoglobulin Fv-fragments R Glockshuber, M Malia, I Pfitzinger, A Plueckthun Biochemistry 29 (6), 1362-1367, 1990 | 712 | 1990 |
| Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage display system A Krebber, S Bornhauser, J Burmester, A Honegger, J Willuda, ... Journal of immunological methods 201 (1), 35-55, 1997 | 664 | 1997 |
| Designing repeat proteins: well-expressed, soluble and stable proteins from combinatorial libraries of consensus ankyrin repeat proteins HK Binz, MT Stumpp, P Forrer, P Amstutz, A Plückthun Journal of molecular biology 332 (2), 489-503, 2003 | 663 | 2003 |
| Reproducibility: Standardize antibodies used in research A Bradbury, A Plückthun Nature 518 (7537), 27-29, 2015 | 589 | 2015 |
| Picomolar affinity antibodies from a fully synthetic naive library selected and evolved by ribosome display J Hanes, C Schaffitzel, A Knappik, A Plückthun Nature biotechnology 18 (12), 1287-1292, 2000 | 568 | 2000 |
| New protein engineering approaches to multivalent and bispecific antibody fragments A Plückthun, P Pack Immunotechnology 3 (2), 83-105, 1997 | 518 | 1997 |
| In-vitro protein evolution by ribosome display and mRNA display D Lipovsek, A Plückthun Journal of immunological methods 290 (1-2), 51-67, 2004 | 502 | 2004 |
| Miniantibodies: use of amphipathic helixes to produce functional, flexibly linked dimeric FV fragments with high avidity in Escherichia coli P Pack, A Plückthun Biochemistry 31 (6), 1579-1584, 1992 | 486 | 1992 |
| Ribosome display efficiently selects and evolves high-affinity antibodies in vitro from immune libraries J Hanes, L Jermutus, S Weber-Bornhauser, HR Bosshard, A Plückthun Proceedings of the National Academy of Sciences 95 (24), 14130-14135, 1998 | 466 | 1998 |
| Designed ankyrin repeat proteins (DARPins): binding proteins for research, diagnostics, and therapy A Plückthun Annual review of pharmacology and toxicology, 489-511, 2015 | 460 | 2015 |
| BIACORE analysis of histidine-tagged proteins using a chelating NTA sensor chip L Nieba, SE Nieba-Axmann, A Persson, M Hämäläinen, F Edebratt, ... Analytical biochemistry 252 (2), 217-228, 1997 | 449 | 1997 |
| Sequence statistics reliably predict stabilizing mutations in a protein domain B Steipe, B Schiller, A Plückthun, S Steinbacher Journal of molecular biology 240 (3), 188-192, 1994 | 430 | 1994 |
Lutz JermutusAstraZenecaVerified email at astrazeneca.com
