Iterative saturation mutagenesis accelerates laboratory evolution of enzyme stereoselectivity: rigorous comparison with traditional methods MT Reetz, S Prasad, JD Carballeira, Y Gumulya, M Bocola Journal of the American Chemical Society 132 (26), 9144-9152, 2010 | 257 | 2010 |
Nitrile hydratases (NHases): at the interface of academia and industry S Prasad, TC Bhalla Biotechnology Advances 28 (6), 725-741, 2010 | 246 | 2010 |
Improved PCR method for the creation of saturation mutagenesis libraries in directed evolution: application to difficult-to-amplify templates J Sanchis, L Fernández, JD Carballeira, J Drone, Y Gumulya, ... Applied microbiology and biotechnology 81, 387-397, 2008 | 155 | 2008 |
A propionitrile-induced nitrilase of Rhodococcus sp. NDB 1165 and its application in nicotinic acid synthesis S Prasad, A Misra, VP Jangir, A Awasthi, J Raj, TC Bhalla World Journal of Microbiology and Biotechnology 23, 345-353, 2007 | 70 | 2007 |
Rhodococcus rhodochrous PA-34: a potential biocatalyst for acrylamide synthesis J Raj, S Prasad, TC Bhalla Process Biochemistry 41 (6), 1359-1363, 2006 | 61 | 2006 |
Nitrile- and Amide-hydrolysing Activity in Kluyveromyces thermotolerans MGBY 37 S Prasad, DR Sharma, TC Bhalla World Journal of Microbiology and Biotechnology 21, 1447-1450, 2005 | 41 | 2005 |
Revisiting the Lipase from Pseudomonas aeruginosa: Directed Evolution of Substrate Acceptance and Enantioselectivity Using Iterative Saturation Mutagenesis S Prasad, M Bocola, MT Reetz ChemPhysChem 12 (8), 1550-1557, 2011 | 40 | 2011 |
Bioconversion of butyronitrile to butyramide using whole cells of Rhodococcus rhodochrous PA-34 J Raj, A Seth, S Prasad, TC Bhalla Applied microbiology and biotechnology 74, 535-539, 2007 | 38 | 2007 |
Purification of a hyperactive nitrile hydratase from resting cells of Rhodococcus rhodochrous PA-34 S Prasad, J Raj, TC Bhalla Indian journal of microbiology 49, 237-242, 2009 | 33 | 2009 |
Bioconversion of benzonitrile to benzoic acid using free and agar entrapped cells of Nocardia Globerula NHB-2 J Raj, N Singh, S Prasad, A Seth, T Bhalla Acta microbiologica et immunologica hungarica 54 (1), 79-88, 2007 | 31 | 2007 |
Bench scale conversion of 3-cyanopyidine to nicotinamide using resting cells of Rhodococcus rhodochrous PA-34 S Prasad, J Raj, TC Bhalla Indian Journal of Microbiology 47, 34-41, 2007 | 20 | 2007 |
Bioconversion of acrylonitrile to acrylamide using polyacrylamide entrapped cells of Rhodococcus rhodochrous PA-34 J Raj, S Prasad, NN Sharma, TC Bhalla Folia microbiologica 55, 442-446, 2010 | 19 | 2010 |
An in silico target fishing approach to identify novel ochratoxin A hydrolyzing enzyme L Dellafiora, C Gonaus, B Streit, G Galaverna, WD Moll, G Vogtentanz, ... Toxins 12 (4), 258, 2020 | 17 | 2020 |
Optimization of culture conditions for hyper production of nitrile hydratase of Rhodococcus rhodochrous PA-34 S Prasad, J Raj, TC Bhalla Indian J Microbiol 44 (4), 251-256, 2004 | 16 | 2004 |
Constitutive acetonitrile hydrolysing activity of Nocardia globerula NHB-2: Optimization of production and reaction conditions H Kumar, S Prasad, J Raj, TC Bhalla CSIR, 2006 | 14 | 2006 |
Directed evolution of enantioselective enzymes: An unceasing catalyst source for organic chemistry MT Reetz, S Wu, H Zheng, S Prasad Pure and Applied Chemistry 82 (8), 1575-1584, 2010 | 12 | 2010 |
Acrylamide synthesis using agar entrapped cells of Rhodococcus rhodochrous PA-34 in a partitioned fed batch reactor J Raj, NN Sharma, S Prasad, TC Bhalla Journal of Industrial Microbiology and Biotechnology 35 (1), 35, 2008 | 12 | 2008 |
Structure and mechanism of benzaldehyde dehydrogenase from Pseudomonas putida ATCC 12633, a member of the Class 3 aldehyde dehydrogenase superfamily MPD Zahniser, S Prasad, MM Kneen, CA Kreinbring, GA Petsko, D Ringe, ... Protein engineering, design and selection 30 (3), 273-280, 2017 | 8 | 2017 |
Ochratoxin A degrading enzymes of Stenotrophomonassp. 043-1a C Gonaus, L Wieland, GG Thallinger, S Prasad FEMS Microbiology Letters 370, fnad028, 2023 | 2 | 2023 |
Toolbox for the extraction and quantification of ochratoxin A and ochratoxin alpha applicable for different pig and poultry matrices B Streit, T Czabany, G Weingart, M Marchetti-Deschmann, S Prasad Toxins 14 (7), 432, 2022 | 2 | 2022 |